Anti Factor IX (human, Christmas factor)
Mouse monoclonal antibody
Cat.No. HYB 133-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Coagulation factor IX is a single chain polypeptide with a molecular mass of 57 kDa. Normal concentrations of factor IX in human plasma is app. 3 µg/ml (1). Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.
Factor IX isolated from human plasma
HYB 133-01 has specificity for native human factor IX
Epitope specificity differs from that of HYB 133-09 as determined by inhibition ELISA.
HYB 133-01 reacts strongly with native factor IX. Strong reaction is seen in ELISA with native factor IX directly coated onto the microtiter well, and also when tested in sandwich ELISA in combination with a polyclonal antibody against factor IX. In Western blotting after SDS-PAGE, HYB 133-01 reacts with factor IX in non-reduced form only.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:6000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
